Mouse IL36G (Interleukin-36 gamma) ELISA Kit (MOFI01421)

The Mouse IL-36 gamma ELISA Kit from Assay Genie enables quantitative measurement of IL-36γ, a pro-inflammatory cytokine of the IL-1 family that signals through IL-36R and amplifies Th17-driven responses. The target has been implicated in psoriasis-like skin inflammation, mucosal barrier defense, and pulmonary challenge models, supporting immunology, dermatology, and infectious disease research.

The assay is configured around a pre-coated 96-well microplate using a sandwich capture approach with colorimetric readout. The standard curve spans a detection range of 7.8 to 500 pg/mL, with a reported sensitivity near 4.69 pg/mL. Each well requires 100 μL of sample; reconstituted standards should be aliquoted and frozen at -20°C to limit freeze-thaw degradation, and unused strips can be returned to the sealed foil pouch with desiccant.

Typical uses include profiling IL-36γ release from stimulated bone-marrow-derived dendritic cells (BMDCs), keratinocyte culture supernatants, and in vivo challenge studies where IL-36R signaling contributes to pathology. For research use only; not intended for diagnostic procedures.