Mouse IL-6R ELISA Kit (MOES01731)

Designed for mouse research, this colorimetric sandwich-ELISA kit from Assay Genie measures IL-6R (interleukin-6 receptor) in serum, plasma and other biological fluids, including cell culture supernatants. The stated sample volume is 100 μL and the assay time is 3 h 30 min. Listed research areas are cancer, immunology and stem cells.

Specifications state a sensitivity of 18.75 pg/mL, a detection range of 31.25-2000 pg/mL and recovery of 80%-120%. The kit recognizes mouse IL-6R, and no significant cross-reactivity or interference between mouse IL-6R and analogues was observed. Reported intra-assay precision CV values are 5.65%, 6.76% and 4.37%, while inter-assay precision CV values are 8.84%, 8.18% and 7.32%. Recovery in EDTA plasma was 90-105% with an average of 97%, and in cell culture media 86-100% with an average of 93%.

The kit comprises a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, antibody and conjugate diluents, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Storage is 2-8°C.