Mouse IL-6 PharmaGenie ELISA Kit (SBRS1443)

The Mouse IL-6 PharmaGenie ELISA Kit from Assay Genie provides quantitative measurement of mouse interleukin-6 in experimental samples. The procedure uses a pre-coated microplate together with a biotinylated detection antibody and streptavidin-HRP conjugate, producing a colorimetric signal proportional to bound analyte.

The protocol specifies a 100 uL sample or standard volume per well, with absorbance read on a standard plate reader.

IL-6 is a pleiotropic cytokine central to inflammatory signaling, B-cell differentiation, and the hepatic acute-phase response. Investigators apply this kit in mouse models of inflammation, sepsis, autoimmune disease, tumor biology, and metabolic dysfunction, where IL-6 concentrations in plasma or serum samples, cell culture supernatant, cell lysate, or tissue lysate provide a quantitative readout of immune activation.

Reagents are shipped refrigerated; standard and detection antibody vials are stored refrigerated and brought to room temperature before use, with stability maintained through the stated shelf life when handled per the supplied protocol. Intended for research use only.