Mouse IL-5 ELISA Kit (MOEB0074)

Assay Genie provides a Sandwich ELISA Kit designed for the quantitative measurement of mouse Interleukin-5 (IL-5) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses a colorimetric detection method and is specific for natural and recombinant mouse Interleukin-5, supporting work within the immunology research area.

Performance characteristics reported by the manufacturer include a detection range of 15.6-1000pg/mL and a sensitivity of 7.8pg/mL. Precision values are 6.9% (Intra CV) and 11.3% (Inter CV). Recovery has been measured in serum (average 113%, range 107-119) and plasma (average 115%, range 109-120). Linearity across dilutions of 1:2, 1:4, 1:8 and 1:16 is reported as follows: serum 104-114%, 97-105%, 100-110%, 103-113%; EDTA plasma 108-117%, 104-114%, 108-118%, 101-110%; heparin plasma 106-116%, 80-89%, 97-110%, 98-111%.

The target analyte, Interleukin-5, is described as a secreted homodimer that is disulfide-linked, with a molecular weight of 15,410 Da (UniProt P04401). Its annotated function is as a factor that induces terminal differentiation of late-developing B-cells to immunoglobulin-secreting cells, with additional roles in eosinophil differentiation, B cell proliferation, and JAK-STAT signalling. The kit is intended for research use only.