Mouse IL-3 Receptor beta PharmaGenie ELISA Kit (SBRS1439)

Investigating signaling through the shared IL-3 receptor β subunit (CD131/CSF2RB) in murine systems is the focus of this immunoassay from Assay Genie. This common signal-transducing chain partners with ligand-specific alpha subunits to assemble functional receptors for IL-3, IL-5, and GM-CSF, and is frequently profiled in research on hematopoietic stem and progenitor cell biology, myeloid lineage commitment, basophil and eosinophil function, and cytokine-driven inflammation in mouse models.

The kit is configured for research use only and is suited to investigators working with cell culture supernatants harvested from mouse specimens or with other mouse-derived sample matrices. Components are supplied as a complete kit in a microplate layout and follow a standard add-sample, add-detection-antibody workflow with absorbance quantification on a conventional plate reader. Researchers frequently integrate data from this assay with orthogonal approaches such as flow cytometry, western blot analysis, or immunohistochemistry to cross-validate receptor expression across tissues and cell populations.

Short-term refrigerated storage of working reagents and protection from light are recommended during the assay window. Each batch is calibrated against an internal reference standard to support inter-lot consistency, and the protocol accommodates sample volumes in the microliter range, allowing multiple replicate wells per experimental condition. Detailed reagent volumes, standard curve concentrations, and recommended dilution factors for atypical matrices are documented in the enclosed product datasheet.