Mouse IL-3 ELISA Kit (MOFI00062)

Designed for the quantitative detection of interleukin-3 in mouse samples, this sandwich ELISA from Assay Genie uses a double-antibody format and has been validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit provides researchers with a sensitive and specific tool for measuring IL-3 in studies of immunology, hematology, and inflammation.

Key analytical specifications include a sensitivity of 9.375 pg/mL and a standard range of 15.625-1000 pg/mL (1pg=170mIU). The colorimetric procedure uses TMB substrate with absorbance read at 450 nm. Reagents are stored at 2-8°C for 12 months. The protocol involves standard and sample incubation at 37°C for 90 minutes, biotin-labeled detection antibody for 60 minutes, HRP-Streptavidin (SABC) for 30 minutes, and TMB color development for 10-20 minutes in the dark before the reaction is stopped. Linearity has been demonstrated at 1:2, 1:4, and 1:8 dilutions for serum, EDTA plasma, and heparin plasma, with recovery data also provided for these sample types.

IL-3 is a key cytokine involved in the regulation of hematopoiesis and immune responses, where it plays a crucial role in the differentiation and proliferation of various immune cells. As such, it is a valuable biomarker for studying immune system disorders, hematological malignancies, and inflammatory diseases. The kit's target protein is referenced under Uniprot P01586, and is also known by synonyms including Hematopoietic growth factor, Mast cell growth factor, MULTI-CSF, multilineage-colony-stimulating factor, Multipotential colony-stimulating factor, and P-cell-stimulating factor.