Mouse IL-23 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit uses a double-antibody format for the quantitative measurement of mouse Interleukin-23 (IL-23). The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The procedure follows a six-step workflow: reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition. Sensitivity is quoted at 9.375 pg/mL with a working range of 15.625-1000 pg/mL. Unopened kit components are stored at 2-8°C for up to 12 months. Spiking recovery averages 93% across serum, EDTA plasma, and heparin plasma samples, and linearity is demonstrated at 1:2, 1:4, and 1:8 dilutions across the same sample types, with reported values falling within the 80-105% range. IL-23 is a key cytokine involved in regulating immune responses and inflammation and is implicated in autoimmune conditions such as inflammatory bowel disease, psoriasis, and rheumatoid arthritis, making this kit a relevant tool for immunology research.