Mouse IL-22 PharmaGenie ELISA Kit (SBRS1430)

Researchers studying cytokine signaling and mucosal immunity use this Assay Genie PharmaGenie kit to measure mouse interleukin-22 concentrations in experimental samples. IL-22 is a member of the IL-10 cytokine family secreted predominantly by Th17 and Th22 lymphocytes, with established roles in epithelial barrier defense, mucosal homeostasis, and inflammatory responses in tissues such as gut, skin, and liver. Studies of colitis, psoriasis-like dermatitis, alcoholic liver disease, and bacterial infection models frequently rely on mouse IL-22 readouts to characterize immune activity.

Validated for cell culture supernatant samples as well as matrices derived from mouse plasma or serum, the kit suits both in vitro and in vivo research workflows. Each package provides pre-coated microplate wells and all reagents needed for ninety-six determinations, supplied as a research-use-only kit. Standard diluent, wash buffer, detection antibody, and substrate components are included alongside a recombinant mouse IL-22 reference standard for standard curve generation.

The protocol follows a typical immunoassay workflow of standard addition, sample incubation, antibody binding, and signal development measured on a standard microplate spectrophotometer. Researchers should bring all reagents to room temperature before use and avoid repeated freeze-thaw cycles of reconstituted standards to preserve reagent activity. Serial dilutions of the supplied reference standard produce a calibration curve that allows interpolation of unknown mouse IL-22 concentrations across experimental conditions.