Mouse IL-1ra (Interleukin 1 Receptor Antagonist) ELISA Kit (MOFI01425)

The Assay Genie Mouse IL-1ra kit quantifies endogenous Interleukin-1 Receptor Antagonist with a microplate-based immunoassay format. Researchers load 50 uL of mouse serum or plasma sample per well alongside a recombinant mouse IL-1ra standard curve spanning 15.6 to 1000 pg/mL. A biotinylated detection antibody paired with streptavidin-HRP produces a measurable signal proportional to bound analyte, and the minimum detectable concentration is approximately 5 pg/mL. The capture antibody is mouse-specific, with no significant cross-reactivity observed against rat or human IL-1ra under standard conditions. Each kit ships with a pre-coated 96-well strip plate, sealed standards, detection antibody, streptavidin-HRP concentrate, wash buffer, TMB substrate, and stop solution. Reconstituted standards and antibody vials should be aliquoted and stored at -20 degrees C to limit freeze-thaw damage, while unopened kit components remain stable for up to six months when refrigerated and protected from light. Typical run time is approximately 3.5 hours from sample addition to plate read, and the protocol is compatible with automated plate washers. The kit is used in inflammatory disease research, including arthritis, sepsis, and acute-phase response studies in murine models.