Mouse IL-17A DIY ELISA Kit

The Mouse IL-17A DIY ELISA Kit from Assay Genie is configured for researchers who coat their own microplates, providing the capture antibody, detection antibody, recombinant mouse IL-17A standard, streptavidin-HRP conjugate, substrate, and wash buffer in bulk quantities sized for processing up to 10 plates per pack. This do-it-yourself workflow suits laboratories running high-volume cytokine profiling projects where plate flexibility, batch customization, or cost-per-sample optimization matter more than pre-packaged convenience.

IL-17A is the signature cytokine of Th17 cells and is widely investigated in mouse models of autoimmune disease, mucosal inflammation, psoriasis-like skin inflammation, rheumatoid arthritis, and host defence against extracellular bacteria and fungi. Quantifying IL-17A in cell culture supernatants and tissue homogenates from treated versus control mice helps characterize Th17 differentiation, gauge the efficacy of candidate therapeutics, and dissect downstream neutrophil recruitment. Reagents are shipped on cold packs; the standard, detection antibody, and streptavidin-HRP should be stored at -20 degrees C to preserve activity, while the capture antibody and diluted buffers can be kept refrigerated at 4 degrees C for short-term use.

Each component is supplied at a working dilution volume calibrated for a 100 µL sample load per well, and the bulk format allows researchers to scale assays from a single plate to the full ten-plate capacity without reordering. The kit is intended for research use only and is not validated for diagnostic procedures.