Mouse IL-17 E PharmaGenie ELISA Kit (SBRS1418)

The Mouse IL-17 E ELISA Kit from Assay Genie quantifies interleukin-25 (IL-25), a Th2-promoting cytokine that drives eosinophil recruitment, goblet cell hyperplasia, and airway hyperresponsiveness in models of allergic asthma and helminth infection. This mouse-specific assay uses a matched-pair antibody configuration with streptavidin-HRP signal generation to measure IL-17E levels across a defined dynamic range, supporting studies in mucosal immunology, atopic dermatitis, and parasitic infection research. Researchers apply the kit to cell culture supernatants from stimulated splenocytes or epithelial cultures, as well as to serum or plasma collected from challenged animals. The microplate format includes removable well strips, lyophilized recombinant standard, pre-coated capture plate, biotinylated detection reagent, and wash buffer concentrate, allowing flexible throughput and replicate layouts. Each well receives a 100 µl sample or standard volume during incubation, with a 50 µl detection antibody addition and final HRP substrate step; wash cycles use 350 µl buffer per well. Working conjugate should be kept refrigerated and shielded from light, while reconstituted standards are best aliquoted and stored frozen to limit freeze-thaw loss of activity.