Mouse IkB alpha (Inhibitory Subunit of NF Kappa B Alpha) ELISA Kit (MOES01330)

Researchers investigating NF-κB signaling in mouse model systems can use this ELISA-based quantitative assay from Assay Genie to measure Inhibitory Subunit of NF Kappa B Alpha (IkB alpha) levels in experimental samples. The 96-assay format supports medium-throughput studies of inflammation, immune cell activation, and cytokine-driven pathway responses, where IkB alpha phosphorylation and degradation serve as canonical readouts of NF-κB pathway engagement.

IkB alpha functions as a cytoplasmic inhibitor that retains NF-κB dimers in an inactive state until upstream kinase signaling triggers its phosphorylation and proteasomal degradation. By quantifying IkB alpha protein levels, researchers can evaluate pathway engagement following stimulation with cytokines, pathogen-associated molecules, or pharmacological agents. The kit is intended for in vitro research use only and supports academic and pharmaceutical laboratory workflows involving mouse-derived specimens.

Each kit contains all components required to run the assay, with protocols calibrated for straightforward integration into standard laboratory procedures. The 96-assay configuration accommodates standard curves, replicate wells, and multiple experimental conditions in a single run. Researchers should consult the supplied protocol insert for details on sample preparation, incubation times, and data interpretation.