Mouse IgG2a (Immunoglobulin G2a) ELISA Kit (MOES01191)

This colorimetric sandwich ELISA from Assay Genie is intended for immunology research and for the quantitative detection of mouse IgG2a in serum, plasma and other biological fluids, including cell culture supernatants. The listed assay time is “3 h 30 min”, with a sample volume of “100 μL” for serum, plasma and other biological fluids.

Reported performance includes a sensitivity of “4.69 ng/mL” and a detection range of “7.81-500 ng/mL”. Recovery is stated as “80%-120%”. Intra-assay precision is given as “CV (%) 4.03 4.16 6.08”, and inter-assay precision as “CV (%) 7.64 7.14 8.34”. Linearity was evaluated in serum, EDTA plasma and cell culture media at dilutions from 1:2 to 1:16. The kit recognises mouse IgG2a in samples, and no significant cross-reactivity or interference between mouse IgG2a and analogues was observed.

The kit includes a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, antibody and HRP conjugate diluents, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Storage is listed at “2-8℃”, with specified components stored at “-20°C, 12 months” and others at “2-8°C, 12 months”.