Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit (MOES01739)

Designed for the accurate quantification of IGFBP5 levels in mouse serum, plasma, and cell culture supernatants, this Sandwich-ELISA from Assay Genie is a highly sensitive and specific assay that delivers reliable and reproducible results for research applications in signal transduction. The colorimetric method uses a biotinylated detection antibody and Avidin-Horseradish Peroxidase (HRP) conjugate, and optical density is measured at 450 nm ± 2 nm.

With a sensitivity of 75 pg/mL and a detection range of 125-8000 pg/mL, the kit supports work on IGFBP5, a key regulator of insulin-like growth factor signaling that influences cell growth, survival, and differentiation and has been implicated in diseases such as cancer, diabetes, and osteoporosis. Sample volume is 100 μL across serum, plasma and other biological fluids, and no significant cross-reactivity or interference between Mouse IGFBP-5 and analogues was observed. Total assay time is 3 h 30 min, with recovery of 80%-120%.