Mouse IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) CLIA Kit (MOES00390)

This chemiluminescence-based CLIA kit from Assay Genie quantitatively measures mouse Insulin-Like Growth Factor Binding Protein 2 in serum, plasma, and other biological fluids using a 96-well microplate format. IGFBP-2 is a key regulator of IGF bioavailability and is implicated in metabolic disorders, tumor biology, and glucose homeostasis research. The assay provides a broader dynamic range and lower detection limit than conventional colorimetric ELISA, supporting applications in knockout mouse models and oncology biomarker validation.

• Detection method: chemiluminescence for enhanced signal-to-noise readout
• Sample volume: typically 50-100 µL per well, run in duplicate
• Format: 96-well plate with pre-coated capture antibody and standards for curve fitting
• Storage: refrigerate kit components at 2-8 °C; avoid repeated freeze-thaw cycles
• For research use only; not intended for diagnostic or clinical applications

Typical workflow includes overnight incubation at 4 °C, washing, detection antibody addition, substrate development, and luminometer readout. Intra-assay and inter-assay precision, plus spike-and-recovery validation in serum matrices, support reliable quantitation across a broad concentration span.