Mouse IFN gamma DIY ELISA Kit

The Assay Genie Mouse IFN gamma DIY ELISA Kit enables researchers to quantify interferon-gamma, a signature Th1 cytokine central to macrophage activation and cell-mediated immunity, in cell culture supernatants and tissue homogenates. The DIY (do-it-yourself) format supplies all critical reagents — biotinylated detection antibody, recombinant standard, streptavidin-HRP, coating buffer, wash concentrate, TMB substrate, and stop solution — so the user coats their own microplate with capture antibody and runs up to 10 plates per kit, providing flexibility for small projects and large screens.

Interferon-gamma (IFN-γ) is produced primarily by activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells, supporting immunology research, vaccine response studies, infection models, and inflammation investigations. The assay delivers a standard curve detection range of approximately 15.6 to 1000 pg/mL with sensitivity near 5 pg/mL, requiring 100 µL of sample per well. Reconstituted standards and diluted samples should be handled promptly, and unopened kit components are stored at refrigerated conditions to preserve antibody activity through the stated shelf life.

Researchers pair this kit with standard ELISA workflow steps including plate coating, blocking, sample incubation, washing, and signal development. The colorimetric readout is captured on a standard plate reader, and the format supports both individual sample testing and batched screening of experimental cohorts in vitro.