Mouse IFN-beta PharmaGenie ELISA Kit (SBRS1392)

The Mouse IFN-beta PharmaGenie ELISA Kit from Assay Genie delivers quantitative measurement of interferon beta, a type I cytokine central to antiviral innate immunity and increasingly studied in autoimmune and neuroinflammation research. The microplate-based sandwich-format assay is calibrated for murine samples with a detection range of 15.6 to 1000 pg/mL and a sensitivity below 5 pg/mL, providing the dynamic range needed to quantify both basal and stimulated cytokine levels in vitro.

Each kit supplies a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and assay diluent. The protocol accommodates cell culture supernatants, plasma, and serum with a 100 uL sample volume per well, and the workflow is typically completed within 3.5 hours at room temperature. After the enzymatic reaction is stopped with the supplied acid solution, absorbance is read on a standard plate reader. Reagents ship on cold packs and should be refrigerated upon arrival; sealed components remain stable for several months when stored properly. For research use only and not intended for diagnostic procedures.