Mouse IFI35 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise quantification of interferon-induced protein 35 (IFI35) in mouse samples. The assay uses a double-antibody sandwich format with colorimetric detection, supporting research into immune regulation, antiviral defence, and inflammatory processes where IFI35 plays a critical role.

Recommended sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit delivers a sensitivity of 46.875 pg/mL with a working range of 78.125–5000 pg/mL, and components should be stored at 2–8°C for 12 months. Linearity across 1:2, 1:4, and 1:8 dilutions was 86–102% / 85–97% / 85–104% for serum, 84–94% / 85–98% / 86–94% for EDTA plasma, and 82–96% / 82–100% / 80–100% for heparin plasma (n = 5). Mean recovery was 94% for serum, 95% for EDTA plasma, and 95% for heparin plasma.

The assay procedure involves equilibrating reagents and TMB substrate to room temperature, followed by a 90-minute primary incubation at 37 °C, a 60-minute biotin-labelled detection antibody incubation, and a 30-minute HRP-Streptavidin step. Colour is developed with TMB substrate in the dark for 10–20 minutes, then stopped and read at 450 nm. Sample preparation guidance is provided for serum (centrifuge at 1000 × g for 20 minutes), plasma (1000 × g for 15 minutes at 2–8 °C), tissue homogenate, cell culture supernatant (2500 rpm for 5 minutes), and cell lysate.