Mouse Hydroxymethylglutaryl-CoA synthase, cytoplasmic (Hmgcs1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA uses colorimetric detection and is designed for the quantitative measurement of mouse Hydroxymethylglutaryl-CoA synthase, cytoplasmic (Hmgcs1) in research samples. The kit is reactive with mouse and has been validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity for natural and recombinant mouse Hmgcs1.

Quoted performance specifications include a detection range of 0.156-10ng/mL and a sensitivity of 0.092ng/mL. HMGCS1 is a cytoplasmic enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, the substrate for HMG-CoA reductase, placing it at a key step of the mevalonate pathway and cholesterol biosynthesis. Dysregulation of HMGCS1 has been implicated in various metabolic disorders, supporting its use as a biomarker within cardiovascular research.

Detection is performed by reading optical density at 450 nm with a microplate reader. Kit components include a dismountable ELISA microplate, lyophilised standard, sample and assay diluents, detection reagents A and B, wash buffer, substrate, stop solution and plate sealers, with storage temperatures across the kit ranging from -20°C to 4°C. The product is for research use only.