Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit

HADHb is a mitochondrial matrix enzyme that catalyzes the penultimate step of fatty acid beta-oxidation, and this colorimetric ELISA Kit from Assay Genie provides researchers with a quantitative immunoassay for measuring the protein in mouse-derived tissue homogenates. The Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) target, also known as SCHAD, is encoded by the HADH gene and has been investigated in mouse models of hyperinsulinemic hypoglycemia and short-chain 3-hydroxyacyl-CoA dehydrogenase deficiency, where loss of function disrupts beta-cell insulin secretion.

Configured for 96 determinations per run, the kit includes a pre-coated microplate, detection antibody, recombinant protein standard, substrate solution, stop solution, wash buffer, and standard diluent. Tissue samples such as liver and skeletal muscle homogenates are accepted alongside additional mouse-derived preparations, with each run producing a standard curve-based readout suitable for studies of mitochondrial fatty acid oxidation, beta-cell metabolism, and inherited metabolic disorders.