Mouse HSF2 (Heat Shock Transcription Factor 2) ELISA Kit (MOES01130)

The Mouse HSF2 (Heat Shock Transcription Factor 2) ELISA Kit from Assay Genie enables quantitative measurement of HSF2 protein in mouse samples. HSF2 is a stress-responsive transcription factor that binds heat shock elements in gene promoters and regulates expression of heat shock proteins, with distinct roles from HSF1 in developmental processes including spermatogenesis, oogenesis, and neuronal differentiation.

Researchers studying cellular stress pathways, reproductive biology, and tumor biology apply this assay to investigate HSF2 expression dynamics under thermal, oxidative, and proteotoxic stress conditions. The kit supports workflows in cell culture supernatant analysis, tissue homogenate profiling, and serum-based biomarker studies using a microplate format with absorbance readouts. Recommended sample volume is 100 uL per well, with standard curves generated from lyophilized recombinant HSF2 across a multi-point range.

For reagent stability, store components refrigerated and protect from light until first use. Allow plates and reagents to equilibrate to room temperature before running the assay, and avoid repeated freeze-thaw cycles of the standard and conjugate solutions. Each pack delivers enough material for one microplate run, including pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, and streptavidin-HRP.