Mouse Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit

Researchers studying immune cell trafficking, lymphocyte homing, and tumor invasion in murine systems can use the Assay Genie Mouse Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit to quantitatively measure HCAM (also known as CD44) protein in mouse research samples. HCAM/CD44 is a widely expressed cell-surface glycoprotein that mediates cell-cell and cell-extracellular matrix interactions through binding to hyaluronic acid and other ligands, playing central roles in inflammatory responses, cancer metastasis, stem cell homing, and tissue remodeling.

This microplate-based immunoassay uses a colorimetric measurement and is supplied in a 96-assay kit format with all necessary reagents, standards, and buffers included. It accepts serum samples, plasma specimens, tissue homogenates, and additional sample matrices, supporting flexible experimental design across cell culture, animal model, and tissue-level studies. The kit is intended for research use only and should be stored refrigerated to preserve antibody binding activity and standard stability through the stated shelf life.

For assay execution, researchers should consult the included protocol for exact standard curve concentrations, recommended sample dilutions, and assay-specific performance characteristics such as detection range and minimum detectable dose. The kit supports parallel processing of multiple samples, making it useful for time-course studies, treatment comparison panels, and screening experiments in mouse models of inflammation, autoimmune disease, fibrosis, and tumor progression. Assay performance and reproducibility depend on proper sample handling, including aliquot preparation and limiting freeze-thaw cycles where applicable.