Mouse Homeobox protein engrailed-2 (En2) ELISA Kit

Assay Genie offers a Sandwich ELISA Kit designed for the accurate quantification of mouse Homeobox protein engrailed-2 (En2) levels in research samples. The kit is reactive with mouse samples and is validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, recognising natural and recombinant mouse En2. Synonyms for the target include Homeobox protein en-2 and En-2.

Performance characteristics include a detection range of 0.312-20ng/mL and a sensitivity of 0.192ng/mL. The target is a nuclear protein with a reported molecular weight of 33,817 Da, catalogued under UniProt P09066 and NCBI Gene ID 13799. In research, En2 is a key homeobox protein involved in the regulation of gene expression and plays a critical role in neural development and patterning, with dysregulation linked to developmental disorders and neurological conditions and making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

For research use only, the kit is supplied with a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents, detection reagents, wash buffer, substrate, stop solution, and a plate sealer. The sandwich ELISA procedure concludes with a colourimetric read at 450 nm using a microplate reader.