Mouse HNF6b (Hepatocyte Nuclear Factor 6 Beta) ELISA Kit (MOES01171)

Assay Genie's ELISA quantifies mouse HNF6b, also known as ONECUT2, a CUT-homeodomain transcription factor essential for hepatocyte differentiation, bile duct morphogenesis, and glucose homeostasis. Researchers apply this kit to mouse models of liver injury, metabolic dysfunction, and biliary atresia, where HNF6b expression shifts during disease progression. Validated for use with mouse serum specimens, plasma, and tissue homogenates, the microplate workflow includes a pre-coated plate, biotinylated detection antibody, recombinant standards, and streptavidin-HRP reagents for absorbance-based quantitation. The standard curve covers 0.156–10 ng/mL with a minimum detectable dose of approximately 0.08 ng/mL. Reagents should be refrigerated upon arrival; aliquot reconstituted standards to limit freeze-thaw cycles and preserve activity over the kit's shelf life. Useful for in vitro hepatic cell line studies and in vivo mouse model research on liver biology and metabolic disease.