Mouse HNF4a/Hepatocyte Nuclear Factor 4 Alpha ELISA Kit

Assay Genie offers a Sandwich ELISA, Double Antibody kit designed for the quantitative measurement of Hepatocyte Nuclear Factor 4 Alpha (HNF4A) in mouse samples, delivering high sensitivity and specificity. HNF4A is a key transcription factor that regulates gene expression in the liver and other organs, playing a critical role in metabolic processes and cell differentiation. Dysregulation of HNF4A has been linked to metabolic disorders, liver diseases, and certain cancers, highlighting its importance as a biomarker for studying these conditions and potential therapeutic targets.

The assay has a sensitivity of 23.438 pg/mL and a standard range of 39.063-2500 pg/mL, and is compatible with cell or tissue lysate and other liquid samples. Reported recovery averages are 96% for serum, 90% for EDTA plasma and 97% for heparin plasma, with linearity across dilutions falling within the 80-105% range. The kit is intended for cell biology research and uses a colorimetric detection method on a pre-coated plate.

The workflow includes a 37°C primary incubation for 90 minutes, detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, and TMB color development in the dark for 10–20 minutes before reading absorbance at 450 nm. Reagents should be stored at 2-8°C for 12 months.