Mouse HNF1b/Hepatocyte Nuclear Factor 1 Beta ELISA Kit

This sandwich ELISA from Assay Genie uses a double antibody format for the detection of HNF1B (Hepatocyte Nuclear Factor 1 Beta) levels in mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The colorimetric assay offers a sensitivity of 4.688 pg/mL across a working range of 7.813-500 pg/mL.

HNF1B is described as a key transcription factor that regulates gene expression during the development and function of the liver, kidney, and pancreas, with dysregulation linked to diabetes, cystic kidney disease, and pancreatic cancer. The product is listed under the Research Area of Epigenetics and Nuclear Signaling, is associated with Uniprot identifier P27889, and kit components should be stored at 2-8°C for 12 months.

Reported validation includes linearity in serum (87-103% at 1:2, 83-100% at 1:4, 85-97% at 1:8), EDTA plasma (85-103%, 84-95%, 82-99%), and heparin plasma (86-104%, 85-98%, 81-96%), with average recoveries of 92% for serum and EDTA plasma and 93% for heparin plasma. The procedure uses a 37°C primary incubation of 90 minutes, followed by a biotinylated detection antibody for 60 minutes, HRP-Streptavidin (SABC) for 30 minutes, TMB substrate in the dark for 10–20 minutes, and absorbance measurement at 450 nm immediately after adding stop solution.