Mouse HMGB4/High mobility group protein B4 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit uses a double-antibody, colorimetric format for the quantitative measurement of High Mobility Group Protein B4 (HMGB4) in mouse samples. With a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL, the assay supports accurate and reproducible quantification for research into DNA-binding, gene expression regulation, inflammation, immune response, and DNA repair.

Validated sample matrices include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with kits stored at 2-8°C for 12 months. The protocol involves equilibrating reagents to room temperature, a 90-minute primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin (SABC) binding, TMB substrate color development in the dark for 10-20 minutes, and absorbance reading at 450 nm immediately after stop solution addition.

Linearity across serum (n=5) is 82-100% at 1:2, 81-100% at 1:4, and 90-98% at 1:8 dilutions; EDTA plasma (n=5) shows 82-98%, 82-93%, and 85-105% at the same dilutions; and heparin plasma (n=5) shows 85-100%, 87-101%, and 82-99%. Recovery averages are 99% for serum, 94% for EDTA plasma, and 96% for heparin plasma.