Mouse Histone H2A.x (H2afx) ELISA Kit

Designed for the quantitative measurement of mouse Histone H2A.X (H2AFX), this Sandwich ELISA from Assay Genie is intended for the sensitive and accurate detection of the target in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The assay is specific for natural and recombinant mouse Histone H2AX and is supplied for research use only, supporting studies in epigenetics and DNA damage biology.

Performance characteristics reported by the manufacturer include a detection range of 31.2–2000 pg/mL and a sensitivity of 15.61 pg/mL. Intra-assay precision is given as 4.3% CV and inter-assay precision as 7.5% CV. Linearity has been evaluated in serum, EDTA plasma and heparin plasma (n=5) at dilutions of 1:2, 1:4, 1:8 and 1:16, with reported ranges of 104–116%, 95–105%, 80–89% and 107–118% in serum; 97–108%, 113–123%, 100–109% and 100–111% in EDTA plasma; and 90–100%, 112–122%, 91–104% and 108–117% in heparin plasma. Spike-and-recovery values are given for serum (average 96%, range 90–102%) and plasma (average 98%, range 92–104%).

Histone H2A.X is a variant histone that replaces conventional H2A in a subset of nucleosomes, contributing to chromatin structure, DNA accessibility and genomic stability. The protein is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double-strand breaks when modified by C-terminal phosphorylation (Uniprot P27661). Subcellular localisation is the nucleus and chromosome.