Mouse Histamine Receptor H3 (HRH3) ELISA Kit

Histamine Receptor H3 (HRH3) is a presynaptic G-protein coupled receptor enriched in the central nervous system, where it functions as both an autoreceptor regulating histamine release and a heteroreceptor modulating dopamine, serotonin, acetylcholine, and noradrenaline signaling. The Assay Genie Mouse HRH3 ELISA Kit provides a quantitative immunoassay for measuring HRH3 protein concentrations in mouse tissue homogenates and related research samples.

The kit uses a matched capture and detection antibody pair with absorbance-based detection read on a standard microplate reader. Each kit contains sufficient reagents for 96 assays and is configured for research use only, supporting investigations in neuropharmacology, sleep-wake regulation, cognitive function, metabolic control, and GPCR signaling research.

Reactivity is limited to mouse samples, reflecting the species-specific antibodies used in the assay format. Tissue samples are prepared by homogenizing fresh or frozen specimens in cold lysis buffer, with clarified supernatant loaded onto the plate following centrifugation for quantitative readout.