Mouse His (Histamine) ELISA Kit (MOFI01440)

Histamine is a key molecule involved in allergic reactions, inflammation, and neurotransmission, making it a vital target for research in immunology, neurology, and inflammation. This competitive ELISA kit from Assay Genie is a sensitive tool for quantifying histamine levels in mouse serum, plasma, and other biological samples, providing accurate and reproducible results across a range of experimental settings.

The assay uses a competitive format with antigen-coated wells and reports a sensitivity of 0.5 ng/mL with a working range of 1.563–100 ng/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The procedure involves biotin-labeled antibody binding, HRP-streptavidin detection with TMB substrate, and absorbance measurement at 450 nm. The sealed kit is stored at 2–8°C and should not be cryopreserved.

Validation studies report linearity across 1:2, 1:4, and 1:8 dilutions: serum 85–97% / 90–100% / 87–90%, EDTA plasma 81–97% / 87–103% / 85–105%, and heparin plasma 92–105% / 86–98% / 85–99%. Recovery averages 94% for serum (range 87–99%), 100% for EDTA plasma (range 85–104%), and 91% for heparin plasma (range 88–104%).