Mouse High mobility group protein B1 (Hmgb1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of High mobility group protein B1 (HMGB1) in mouse samples. It uses a colorimetric detection method and recognises natural and recombinant mouse HMGB1 with reactivity confirmed for mouse-derived specimens.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Reported performance characteristics are a detection range of 0.156-10ng/mL, a sensitivity of 0.082ng/mL, an intra-assay CV of 7.1% and an inter-assay CV of 9.2%. Linearity has been assessed in serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16, and recovery averages 102% (range 96-108%) in serum and 104% (range 98-110%) in plasma. The kit is listed under the Epigenetics research area.

HMGB1 is described as a key protein involved in inflammatory responses, with roles reported in sepsis, cancer and autoimmune disorders, making the kit suitable for studies of inflammation, immune responses and disease pathogenesis in mouse models.