Mouse Hepcidin ELISA Kit

A high-quality sandwich ELISA from Assay Genie, this kit is designed for the precise quantitative measurement of hepcidin in mouse samples. Hepcidin is described as a key regulator of iron metabolism, controlling the absorption and distribution of iron in the body, and its dysregulation has been linked to conditions such as anemia, hemochromatosis, and various inflammatory disorders. The kit provides a reliable and efficient method for quantifying hepcidin levels in biological samples, supporting research into iron homeostasis and related diseases.

The assay uses a double-antibody sandwich format with colorimetric detection. It has a sensitivity of 1.875 ng/mL and a measuring range of 3.125-200 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for 12 months. The associated UniProt entry is Q9EQ21.

Validation data demonstrate consistent performance across sample matrices. Linearity at 1:2, 1:4, and 1:8 dilutions: serum (n=5) 91-105%, 86-99%, 85-104%; EDTA plasma (n=5) 85-101%, 83-97%, 82-101%; heparin plasma (n=5) 88-97%, 85-96%, 83-100%. Recovery ranges with averages: serum 86-103% (96%), EDTA plasma 89-100% (93%), heparin plasma 91-105% (97%). The protocol involves a 90-minute primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-streptavidin binding, TMB color development, and absorbance reading at 450 nm.