Mouse Heparin-binding growth factor 1 (Fgf1) ELISA Kit

The Assay Genie Mouse Heparin-binding growth factor 1 (Fgf1) ELISA Kit is a colorimetric Sandwich ELISA designed for the quantitative measurement of mouse FGF1. The assay uses pre-coated microplate wells with specific capture antibodies, combined with biotinylated detection antibodies and an Avidin-Horseradish Peroxidase (HRP) conjugate, with absorbance read spectrophotometrically at 450 nm ± 2 nm.

It is validated for use with serum, plasma, saliva, urine, cell culture supernatant, tissue samples, and other related supernatants. The assay has a detection range of 31.2-2000pg/mL and a sensitivity of 15.6pg/mL. Reported precision is an intra-assay CV of 5.1% and an inter-assay CV of 7.2%, with specificity demonstrated for natural and recombinant mouse Fibroblast growth factor 1. Kit components should be stored at -20°C, and the product is intended for research use only.

FGF1, also known as acidic fibroblast growth factor (aFGF, HBGF-1), is a key signaling molecule involved in the regulation of cell survival, cell division, angiogenesis, cell differentiation, and cell migration. It acts as a ligand for FGFR1 and integrins, and is implicated in biological processes such as wound healing, tissue repair, and development, making it a target of interest in diseases including cancer, cardiovascular disorders, and diabetes.