Mouse Hcn2 ELISA Kit

Assay Genie offers a sandwich, double-antibody ELISA kit designed for the quantitative measurement of HCN2 (hyperpolarization-activated cyclic nucleotide-gated channel 2) in mouse samples. The target protein is involved in regulating neuronal excitability and cardiac pacemaker activity, and its dysregulation has been linked to neurological disorders and cardiac conditions. Annotated synonyms for the target include Hcn2, Brain cyclic nucleotide-gated channel 2, BCNG-2, and BCNG2.

Validated for use with mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, the assay reports a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Linearity spans 82–104% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, while recovery averages are 93% (Serum), 97% (EDTA Plasma), and 92% (Heparin Plasma). The procedure uses a biotin-labeled detection antibody, an HRP-Streptavidin conjugate, and a TMB substrate, with absorbance read at 450 nm. The kit is intended to be stored at 2–8°C for 12 months.

Research area: Signal Transduction. Associated UniProt identifier: O88703.