Mouse HABP1/Hyaluronan Binding Protein 1 ELISA Kit

A colorimetric Sandwich ELISA Kit from Assay Genie, employing a double antibody format, is designed for the precise measurement of HABP1 (Hyaluronan Binding Protein 1) in mouse samples. The kit is reactive with mouse and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

HABP1 binds hyaluronan, a key component of the extracellular matrix, and contributes to cell migration, proliferation, and tissue remodeling. Its dysregulation has been linked to cancer, inflammation, and fibrosis, making it a useful biomarker for investigating these conditions. The kit is stored at 2–8°C for 12 months. Reported linearity at 1:2 dilution spans 87–103% for serum, 86–102% for EDTA plasma, and 88–101% for heparin plasma, while recovery averages reach 95% (serum), 94% (EDTA plasma), and 96% (heparin plasma).

The procedure comprises equilibration of reagents, a 37°C primary incubation of 90 minutes, a 60-minute biotin-labeled detection antibody step, a 30-minute HRP-Streptavidin incubation, TMB color development in the dark for 10–20 minutes, and absorbance reading at 450 nm following stop solution addition.