Mouse H-2 class II histocompatibility antigen gamma chain (Cd74) ELISA Kit

Assay Genie provides a Sandwich ELISA kit for the quantitative detection of the mouse H-2 class II histocompatibility antigen gamma chain (CD74), intended for research use only. The assay is reactive with mouse samples and has been validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Specificity covers the natural and recombinant form of the mouse target, and the listed research area is cancer.

Detection is by ELISA, with a quoted detection range of 0.156-10ng/mL and a sensitivity of 0.066ng/mL. The reported intra-assay CV is 0.0% and the inter-assay CV is 0.0%. Recovery in serum is given as an average of 80% (range 80-80%) and in plasma as an average of 80% (range 80-80%). The target protein plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. The protein is organised as a nonamer composed of three alpha/beta/gamma heterotrimers and is a single-pass type II membrane protein. Synonyms include Ia antigen-associated invariant chain, MHC class II-associated invariant chain, Ii, and CD74, and the associated UniProt entry is P04441.