Mouse Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit

Guanylin, the endogenous peptide encoded by GUCA2A, binds intestinal guanylate cyclase C (GUCY2C) and triggers the cGMP signaling pathway that governs epithelial sodium and chloride transport, gut motility, and mucosal fluid balance. This Assay Genie Mouse Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit enables researchers studying gastrointestinal hormone biology, models of secretory diarrhea, intestinal inflammation, and metabolic regulation in mouse models. The microplate format provides pre-coated wells, detection antibody, standards, and substrate reagents, with all components supplied in individual vials and sufficient for 96 assays. Standard sample volume is approximately 50 uL per well, and the assay provides quantitative measurement of mouse guanylin with minimal cross-reactivity to related intestinal peptides. Reconstituted reagents should be kept on ice and protected from light, and unused aliquots should be frozen at -20°C to limit repeated freeze-thaw cycles. The assay is intended for research use only, and quantitative readouts should be interpreted against the standard curve generated on every plate.