Mouse Guanine nucleotide-binding protein G (I)/G (S)/G (T)subunit beta-3 (Gnb3) ELISA Kit

Assay Genie supplies this sandwich ELISA kit for the quantitative measurement of mouse guanine nucleotide-binding protein G (I)/G (S)/G (T) subunit beta-3 (Gnb3), also known as transducin beta chain 3. The kit is intended for research use only and targets a signalling protein involved in intracellular pathways such as neurotransmission, cell growth and metabolism. Dysregulation of GNB3 has been implicated in conditions including hypertension, obesity and insulin resistance.

Performance characteristics stated in the product datasheet include a detection range of 0.78–50 ng/mL and a sensitivity of 0.425 ng/mL. Reported precision values are 6.9% intra-assay CV and 8.8% inter-assay CV. Linearity has been evaluated in serum (85–113% across 1:2 to 1:16 dilutions), EDTA plasma (82–119%) and heparin plasma (88–124%). Mean recovery is 95% for serum and 97% for plasma, with corresponding ranges of 89–101% and 91–103%.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, with specificity stated as natural and recombinant mouse GNB3. Supporting molecular details listed are UniProt accession Q61011, NCBI Gene ID 14695 and a reported molecular weight of 37,240 Da. The protein belongs to the WD repeat G protein beta family and acts as the beta subunit of a heterotrimeric G protein involved in transmembrane signalling, where it interacts with RASD2 and is categorised in the research area of Neurosciences.