Mouse GSK3 beta (Glycogen Synthase Kinase 3 Beta) ELISA Kit (MOES01644)

Glycogen Synthase Kinase 3 Beta (GSK3β) is a constitutively active serine/threonine kinase that phosphorylates glycogen synthase to inhibit glycogen biosynthesis, and it functions as a key regulator of the Wnt/β-catenin, PI3K/AKT, and insulin signaling pathways. Accurate measurement of endogenous GSK3β protein levels in mouse samples supports research on glucose homeostasis, neurobiology (including tau phosphorylation biology relevant to Alzheimer's models), inflammatory signaling, and Wnt-driven oncogenesis.

This colorimetric immunoassay from Assay Genie is supplied in a microplate configuration providing 96 assays per kit and is intended for research use only. Reagents are provided for establishing a standard curve and for quantifying GSK3β in mouse specimens such as tissue homogenates, cell lysates, or cleared supernatants from cultured cells. The kit ships with all components required to perform the protocol as written; laboratories generally prepare samples by homogenization in an appropriate lysis buffer followed by clarification prior to loading onto the plate.