Mouse Growth/differentiation factor 5 (Gdf5) ELISA Kit

A high-quality sandwich ELISA from Assay Genie, designed for the sensitive and specific detection of mouse Growth/differentiation factor 5 (GDF5). The kit accurately quantifies GDF5 in mouse samples and is validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, delivering reliable and reproducible results across a variety of research applications.

GDF5 is a key regulator of bone and joint development, playing a crucial role in skeletal growth and differentiation. Dysregulation of GDF5 has been associated with various musculoskeletal disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions. The assay is specific for natural and recombinant mouse GDF5 and targets a secreted cytokine involved in chondrogenic differentiation through BMPR1B/BMPR1A-mediated SMAD signaling.

Reported performance includes a detection range of 31.2–2000 pg/mL and a sensitivity of 15.7 pg/mL. The intra-assay CV is 8.3% and the inter-assay CV is 9.8%. Recovery averages 109% (range 103–115%) in serum and 111% (range 105–117%) in plasma, with linearity validated for serial dilutions (1:2, 1:4, 1:8, 1:16) in serum, EDTA plasma, and heparin plasma samples. For research use only.