Mouse GRN (Granulin) ELISA Kit (MOES01100)

Assay Genie offers a sandwich-ELISA kit for the colorimetric measurement of mouse GRN (granulin) in serum, plasma, cell culture supernatants and other biological fluids. Sensitivity is 0.94 ng/mL, the detection range is 1.56-100 ng/mL, the stated sample volume is 100 μL and the assay time is 3 h 30 min. Overall recovery is listed as 80%-120%.

Specificity data state that the kit recognizes mouse GRN and that no significant cross-reactivity or interference between mouse GRN and analogues was observed. In the listed recovery testing, EDTA plasma gave a range of 90-103 with an average recovery of 97%, while cell culture media gave 84-98 with an average recovery of 91%. Reported intra-assay CV values are 6.03%, 5.29% and 6.06%, and inter-assay CV values are 8.65%, 7.3% and 6.41%. Linearity data are listed for serum, EDTA plasma and cell culture media.

Supplied components include a dismountable micro ELISA plate, reference standard and sample diluent, concentrated biotinylated detection antibody, concentrated HRP conjugate, biotinylated detection antibody diluent, HRP conjugate diluent, wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Listed research areas are cancer, immunology, neuroscience and signal transduction.