Mouse Granzyme B (GZMB) ELISA Kit

Granzyme B is a serine protease secreted by cytotoxic T lymphocytes and natural killer cells that enters target cells via perforin pores and triggers apoptosis through caspase activation. This colorimetric ELISA kit from Assay Genie quantifies mouse Granzyme B in research samples including plasma, serum, and tissue homogenates, supporting immunology studies of CTL and NK cell effector function. Applications include investigations of viral clearance, tumor immune surveillance, transplant rejection, and autoimmune disease models where granzyme release serves as a biomarker of cytotoxic activity. The kit includes pre-coated microplate strips, biotinylated detection antibody, streptavidin-HRP reagents, recombinant mouse GZMB standard, and TMB substrate. Researchers typically add 50-100 uL of diluted sample per well and capture absorbance readings on a standard microplate reader. The kit is intended for research use only; unopened components should be kept refrigerated, and reconstituted standards aliquoted and frozen to limit freeze-thaw cycles.