Mouse Granulin ELISA Kit

An immunoassay kit from Assay Genie is designed to detect and measure granulin levels in mouse samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid sample types. Granulin is a protein associated with cell growth, wound healing, and inflammation, and it has been implicated in cancer, neurodegenerative disorders, and inflammatory conditions. Studying granulin in mouse models can offer insights into disease mechanisms and potential therapeutic targets.

Manufacturer-reported performance includes a sensitivity of 0.281 ng/mL and a measurement range of 0.469-30 ng/mL. Linearity in serum (n=10) is reported as 85-103% at 1:2, 86-102% at 1:4, and 85-102% at 1:8 dilution; EDTA plasma (n=10) shows 82-94%, 90-98%, and 88-101% across the same dilutions; heparin plasma (n=10) shows 93-100%, 81-98%, and 84-99%. Average recovery is 95% for serum, 94% for EDTA plasma, and 94% for heparin plasma. The associated UniProt entry is P28798 (KEGG K23879; GeneID 14824), with synonyms GRN, Granulins, PC cell-derived growth factor (PCDGF), Proepithelin, and PEPI.

Storage is 2-8°C when sealed, with no cryopreservation, per the manufacturer. The procedure relies on TMB substrate colour development with absorbance read at 450 nm, using a biotin-labelled antibody and an HRP-streptavidin conjugate. Sample preparation guidance covers serum, plasma, cell culture supernatant, cell lysate, and tissue homogenate, with specified centrifugation conditions for each matrix.