Mouse GR beta (glucocorticoid receptor beta) ELISA Kit (MOES01075)

This quantitative immunoassay from Assay Genie is engineered around a double-antibody recognition scheme in which a pre-titered capture antibody is immobilized on each well and a biotinylated reporter antibody, bridged by HRP-streptavidin and a TMB chromogen, produces the readout. The reagent discriminates the GRβ splice variant of the NR3C1 gene, an isoform that lacks the hormone-binding pocket of GRα and instead operates as a constitutive repressor of glucocorticoid-driven gene expression. Because GRβ upregulation has been linked to steroid-unresponsive airway disease, T-cell leukemia resistance to dexamethasone, and altered hypothalamic-pituitary-adrenal axis feedback in chronic stress models, the kit is widely used in pulmonary, immunological, and behavioral endocrinology laboratories. Reported intra-assay variation is consistent with research-grade ELISA performance, and the assay has been documented to perform acceptably across anticoagulated blood fractions and solubilized cell preparations when samples are prepared according to the included technical insert. For projects requiring multiple freeze-thaw cycles, the kit recommends aliquoting thawed standards and buffers into single-use vials before refreezing at -20°C; the coated plate itself should be returned to its desiccated foil pouch and refrigerated at 4°C between runs.