Mouse gp130 (Glucoprotein 130) ELISA Kit (MOES01077)

Mouse models of cytokine-driven inflammation frequently require precise quantification of glycoprotein 130, the IL-6 family signal transducer encoded by Il6st that drives JAK/STAT, MAPK, and PI3K/AKT activation downstream of ligands such as IL-6, IL-11, leukemia inhibitory factor, and oncostatin M. The Assay Genie kit addresses that need with a research-use-only immunoassay workflow built around pre-coated plate wells, matched detection reagents, a recombinant calibrator, and chromogenic substrate readable on standard benchtop plate readers.

Working volumes are scaled for routine laboratory throughput, and the assay accommodates mouse-derived circulatory specimens along with cell culture supernatant and tissue homogenate samples collected from experimentally treated mice. Reconstituted standards and concentrated detection antibody are stored refrigerated to preserve binding activity, and the working substrate solution should be kept protected from light until addition. Curve fitting is performed with the included analysis parameters, and results are expressed against the supplied calibrator so investigators can compare glycoprotein 130 concentrations across treatment cohorts.

Downstream uses include tracking circulating soluble receptor levels in acute-phase response studies, evaluating pharmacodynamic effects of cytokine-receptor blockade in autoimmune and oncology models, and supporting neuroscience work where gp130-mediated trans-signaling has been implicated in neuroinflammation. Pairing the kit with phospho-STAT3 western blots, quantitative PCR for Il6st transcripts, or immunohistochemistry on matched tissue sections helps confirm that changes in circulating receptor reflect, or precede, downstream signaling alterations in target organs.