Mouse Glycoprotein A33 (GPA33) ELISA Kit

GPA33 is a transmembrane glycoprotein of the immunoglobulin superfamily that is predominantly expressed in normal gastrointestinal epithelium and frequently retained in colorectal adenocarcinomas, making it a long-standing focus of intestinal immunology and oncology research. This quantitative immunoassay from Assay Genie is configured for research use with mouse-derived samples and is supplied as a complete 96-assay kit containing all reagents, coated plates, and buffers required to run the full plate. The assay accommodates tissue homogenates and cell lysates prepared from murine intestinal or tumor tissue, supporting studies that examine A33 antigen expression, antibody-target engagement, or treatment response in colitis and colorectal cancer models. Detection relies on an enzymatic reporter system with absorbance measurement on a standard microplate reader, eliminating the need for specialized instrumentation beyond what most immunology laboratories already operate. Researchers should consult the enclosed protocol for recommended dilutions of lysate samples and for guidance on aliquoting and storage of kit components between uses, including handling of any unused wells for repeat measurements on the same plate.