Mouse Glutathione Synthetase (GSS) ELISA Kit

Quantitative determination of glutathione synthetase in mouse research specimens is the purpose of this ELISA Kit from Assay Genie, providing a complete reagent set for 96 individual reactions. Glutathione synthetase (GSS) catalyzes ATP-dependent ligation of γ-glutamylcysteine and glycine to form glutathione, the dominant intracellular thiol antioxidant that buffers cells against reactive oxygen species.

Investigators in redox biology, toxicology, and hepatic pathophysiology use this assay to monitor enzyme expression across genetic knockout, dietary, and pharmacological disease models. Compatible sample inputs include clarified tissue lysates, cell culture supernatants, and clarified body fluids collected from murine sources.

Each kit ships with a pre-coated microplate, lyophilized recombinant standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, stop reagent, and sample dilution buffer. Reconstitution volumes, dilution schemes, and incubation times are detailed in the lot-specific insert.

Long-term storage of unopened kit components should follow the cold-chain instructions printed on each vial. Working dilutions should be prepared fresh before each run, and HRP conjugates must be protected from prolonged light exposure. Aliquot the reconstituted standard into single-use vials to limit repeated freeze-thaw cycles across extended study timelines.