Mouse Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit

The Mouse Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit from Assay Genie provides researchers with a quantitative tool to measure GSTa3 protein abundance in murine samples. GSTa3 is an alpha-class glutathione S-transferase isozyme (~25 kDa) that catalyzes the conjugation of reduced glutathione to electrophilic substrates, supporting Phase II biotransformation and protecting tissues from oxidative damage. Beyond xenobiotic detoxification, GSTa3 displays steroid isomerase activity and is frequently studied in hepatic drug metabolism, environmental toxicology, and models of chemically induced organ injury.

Investigators examining hepatic responses to toxicant exposure, oxidative stress biomarkers, or glutathione-dependent signaling in inflammation and cancer biology will find this microplate-format immunoassay valuable. The kit is supplied as a complete package with wells pre-coated with capture antibody, matched detector antibodies, calibrated reference standards, and buffer reagents for 96 determinations, supporting direct quantification across clarified sample matrices. The antibody pair is calibrated specifically for the murine GSTa3 isoform and exhibits minimal cross-reactivity with other rodent alpha-class GST isozymes.

For best results, store unopened components refrigerated and protect reconstituted reagents from repeated freeze-thaw cycles. The assay is intended for research use only and is not validated for diagnostic procedures. With a small sample volume per well, the protocol supports high-throughput screening of murine cohorts in academic and pharmaceutical research environments.