Mouse Glutamine synthetase (Glul) ELISA Kit

Assay Genie provides a Sandwich ELISA configured for the quantitative detection of mouse Glutamine synthetase (Glul), supplied as a colorimetric immunoassay intended for research use only. The kit is reactive with mouse samples and is validated for serum and plasma, while the manufacturer also lists tissue homogenates, cell culture supernates and other biological fluids as acceptable matrices. Specificity is reported for both natural and recombinant mouse glutamine synthetase, and the kit is positioned for work in the neurosciences research area.

Manufacturer-quoted performance figures include a detection range of 15.6-1000 pg/ml and a sensitivity of 7.94 pg/mL. Precision is given as an intra-assay CV of 5.8% and an inter-assay CV of 8.4%. Linearity has been characterised at dilutions of 1:2, 1:4, 1:8 and 1:16 in serum (85-95%, 107-117%, 83-93%, 92-102%), EDTA plasma (98-108%, 99-109%, 83-95%, 104-114%) and heparin plasma (92-102%, 90-100%, 104-114%, 88-96%). Spike-and-recovery averages are 107% (range 101-113%) in serum and 109% (range 103-115%) in plasma.

Glutamine synthetase catalyses the ATP-dependent conversion of glutamate and ammonia to glutamine. The source describes a tissue-dependent role, regulating toxic ammonia and converting neurotoxic glutamate to harmless glutamine in the brain and contributing to ammonia removal in the liver, with dysregulation linked to neurological and metabolic disorders. The target protein corresponds to UniProt P15105 with a reported molecular weight of 42,120 Da, localising mainly to the cytosol with a fraction associated with the cell membrane.