Mouse Glutamate decarboxylase 1 (Gad1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise quantitative detection of mouse Glutamate decarboxylase 1 (GAD1), also known as GAD-67 or the 67 kDa glutamic acid decarboxylase isoform, in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay detects natural and recombinant mouse GAD1, offering a detection range of 0.39-25 ng/mL and a sensitivity of 0.196 ng/mL. Intra-assay precision is 6.0% and inter-assay precision is 8.3%.

GAD1 catalyses the production of the neurotransmitter gamma-aminobutyric acid (GABA) from glutamate and functions as a homodimer, playing a crucial role in regulating neuronal excitability in the brain. Dysregulation of this enzyme has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic approaches. The kit is specific to mouse samples, with applications in cell biology research, and is intended for research use only.

Validation data show linearity ranging from 83-124% across serum, EDTA plasma and heparin plasma at dilutions from 1:2 to 1:16. Recovery averages 111% for serum (range 105-117%) and 113% for plasma (range 107-119%). The associated UniProt entry is P48318 and the molecular weight is 66,648 Da.